Table 1 Primers used in ARMS and realtime PCR for IL23R gene rs1884444 genotyping and relative expression
|  | Primer Name | Primer Sequence | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
ARMS-PCR | Common Forward | 5’- TCCTCCCTAATCAAAGGTTCCCATC-3’ | 61.88 | 181 |
WT Reverse Primer | 5’- CTATTACTGCATCCCATTGAATAGTGATA-3’ | 59.27 | ||
Mutant Reverse Primer | 5’- CTATTACTGCATCCCATTGAATAGTGATC-3’ | 60.18 | ||
GH (5F) | 5’- TGCCTTCCCAACCATTCCCTTA-3’ | 61.65 | 434 | |
GH (5R) | 5’- CCACTCACGGATTTCTGTTGTGTTTC-3’ | 62.85 | ||
Sequencing Primer | 5’- GGGCTGTCTAGAAGGGAAATTTGAG-3’ | 61.38 | 431 | |
Expression | IL-23R (F) | 5’-ACATGCTTCTATGTACTGCACTG-3’ | 58.81 | 184 |
IL-23R (R) | 5’-TGTGTCTATGTAGGTGAGCTTCC-3’ | 59.55 | ||
β2M (F) | 5’-CCTGAATTGCTATGTGTCTGGG-3’ | 59.05 | 244 | |
β2M (R) | 5’-TGATGCTGCTTACATGTCTCGA-3’ | 59.83 |