Fig. 1

ED-A fibronectin production by OA FLSs. a and b. Representative confocal microscopy images of αSMA (red) and ED-A fibronectin (green) in OA FLS cultures incubated with medium, TGFβ, TNFα, LPS, or IL-6 (n = 3). No staining was seen using isotype control antibodies. c ED-A fibronectin was expressed as a ratio of ED-A fibronectin positive cells divided by the total cell count. Data were log transformed and analyzed with the paired t-test. Bars indicate the median and whiskers indicate the IQR. * P < 0.05