A proteomic analysis of serum-derived exosomes in rheumatoid arthritis

BMC Rheumatology

Table 3 Proteins identified by mass spectrometry

Spot ID	Observed		Fold difference				Protein	Theoretical		Masscot Score	Coverage (%)	Confirmed Sequences (Masscot ion score)	Search log
	MW	pI	wRA		OA	HL		MW	pI
	MW	pI	aRA	iRA	OA	HL		MW	pI
208	90	5.3	1.4*		1.0	1.0	Pro-neuregulin-3, membrane-bound isoform	78	7.8	61	12	³⁸⁸QAKQIQEQLK³⁹⁷(19)	38,220
208	90	5.3	1.4*		1.0	1.0	NRG3_HUMAN	78	7.8	61	12	³⁸⁸QAKQIQEQLK³⁹⁷(19)	38,220
236	87	5.1	1.1		1.3*	1.0	Cathepsin F	53	8.5	26	3	¹⁰³TLLCSFQVLDELGRHVLLR¹²¹(26)	38,210
236	87	5.1	1.1		1.3*	1.0	CATF_HUMAN	53	8.5	26	3	¹⁰³TLLCSFQVLDELGRHVLLR¹²¹(26)	peptide summary
325	65	5.1	0.9		0.7*	1.0	Ig alpha-2 chain C region	37	5.7	132	10	²⁵¹WLQGSQELPR²⁶⁰(53)	38,207
325	65	5.1	0.9		0.7*	1.0	IGHA2_HUMAN	37	5.7	132	10	²⁷⁰QEPSQGTTTFAVTSILR²⁸⁶(70)	38,207
370	53	5.1	1.3*	0.9	0.9	1.0	Alpha-1-antitrypsin	47	5.4	170	33	⁵⁰ITPNLAEFAFSLYR⁶³(79)	38,190
370	53	5.1	1.3*	0.9	0.9	1.0	A1AT_HUMAN	47	5.4	170	33	²¹⁶GKWERPFEVK²²⁵(10)	38,190
494	40	7.0	0.7*	0.9	0.9	1.0	Keratin, type II cytoskeletal 1	66	8.2	62	9	²¹²WELLQQVDTSTR²²³(14)	38,206
494	40	7.0	0.7*	0.9	0.9	1.0	K2C1_HUMAN	66	8.2	62	9	²²⁴THNLEPYFESFINNLR²³⁹(41)	38,206
564	24	5.2	6.3*	1.2	1.1	1.0	Toll-like receptor 3	104	6.7	68	19	⁵³²LEILDLQHNNLAR⁵⁴⁴(7)	38,215
564	24	5.2	6.3*	1.2	1.1	1.0	TLR3_HUMAN	104	6.7	68	19	⁵³²LEILDLQHNNLAR⁵⁴⁴(7)	38,215

MW molecular weight, wRA whole RA, aRA active RA, iRA inactive RA, OA osteoarthritis, HL healthy donor
Fold differences are shown with the intensity of the protein spots in HLs defined as 1.0. Asterisks indicate that the spot intensity is significantly different (p < 0.05) from that in HLs

ISSN: 2520-1026