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Fig. 5 | BMC Rheumatology

Fig. 5

From: Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis

Fig. 5

Effect of ED-A fibronectin on TNFα production. a-c RAW 264.7 cells incubated untreated (UT), with LPS (100 ng/ml), or with recombinant ED-A fibronectin (10 μg/ml). TNFα concentration in supernatants of cultures after 24 h. a Effect of ED-A fibronectin (n = 9). b Cells cultured with or without polymyxin B at 15 μg/ml (PmB) (n = 3). c ED-A fibronectin with or without pretreatment with boiling for 30 min (Heat) (n = 3). d ED-A fibronectin with or without pretreatment with proteinase K at 20 μg/ml (pK) (n = 3). Data were analyzed with the paired t-test. Bars indicate the median and whiskers indicate the IQR. ** P < 0.01

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