Fig. 5
From: Eosinophils in anti-neutrophil cytoplasmic antibody associated vasculitis
Intracellular reactive oxygen species (ROS) production in eosinophils was determined by Phagoburst kit and the produced ROS upon activation with phorbol 12-myristate 13-acetate (PMA) or opsonized E. coli were measured using flow cytometry and shown as geometric mean fluorescence intensity (MFI). The gating strategy and a typical pattern is shown in Additional file 1. The two-sided Mann-Whitney test was used to calculate the level of significance. Horizontal lines represent the median value of each dataset. Eosinophils from patients with anti-neutrophil cytoplasmic antibodies associated vasculitides (AAV) produced fewer ROS than eosinophils from healthy blood donors