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Fig. 4 | BMC Rheumatology

Fig. 4

From: The antifibrotic drug pirfenidone inhibits spondyloarthritis fibroblast-like synoviocytes and osteoblasts in vitro

Fig. 4

Effects of PFD on secretion of regulators of bone metabolism by SpA FLSs and osteoblast mineralization. a-b Column bar graph of DKK-1 and OPG secretion by SpA FLSs untreated (UT) and stimulated with TGFβ, TNFα, IFNγ or all three cytokines (All) with or without pirfenidone (PFD) (n = 4). Data were normalized to untreated cultures without PFD (ratio), log-transformed and analyzed with the paired t-test. c Column bar graph of hydroxyapatite deposition by Saos-2 cells incubated with PFD (triplicates). Saos-2 cells were incubated with increasing concentrations of PFD for 14 days. Data were normalized to untreated cultures without PFD (ratio), log-transformed and comparisons were made with the paired t-test or the repeated measures one-way ANOVA depending on the number of groups. Boxes and bars indicate median and IQR. * p < 0.05. ** p < 0.01. *** p < 0.005

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